Sensitive Analysis of Retinyl Esters by Isocratic Adsorption Chromatography
نویسندگان
چکیده
منابع مشابه
Retinol and retinyl esters: biochemistry and physiology.
By definition, a vitamin is a substance that must be obtained regularly from the diet. Vitamin A must be acquired from the diet, but unlike most vitamins, it can also be stored within the body in relatively high levels. For humans living in developed nations or animals living in present-day vivariums, stored vitamin A concentrations can become relatively high, reaching levels that can protect a...
متن کاملGradient LC analysis of herbicides and polyaromatic hydrocarbons by isocratic capillary electro- chromatography
Capillary electrochromatography (CEC) combines the separation principle of HPLC (partitioning between mobile and stationary phases) with the high efficiency of capillary electroseparation methods. In CEC the electroosmotic flow (EOF) inherent in capillary electrophoretic separations is used to transport solute and mobile phase through a packed capillary column. The properties of the EOF provide...
متن کاملDetermination of retinyl esters and retinol in serum or plasma by normal-phase liquid chromatography: method and applications.
Retinol and retinyl esters are measured in serum or plasma samples by gradient, normal-phase, adsorption "high-performance" liquid chromatography, with ultraviolet detection at 325 nm. The four major circulating retinyl esters in humans (esters of palmitate, stearate, oleate, and linoleate) are coeluted as a single peak. Retinyl acetate is included as an internal standard, to correct for variab...
متن کاملSimultaneous determination of retinol and retinyl esters in serum or plasma by reversed-phase high-performance liquid chromatography.
We propose a single-run liquid-chromatographic determination, with ultraviolet detection at 330 nm, for serum retinol and retinyl esters. The vitamin A derivatives are extracted according to the Bligh-Dyer procedure. With 200 microliter or serum, the lower detection limit is 50 microgram/liter for retinol and about 100 microgram/liter for retinyl esters. Within-run precision (CV) was 2.3% for r...
متن کاملRetinyl esters are hydrolyzed in early endosomes of J774 macrophages.
The aim of the current study was to identify the subcellular compartment(s) responsible for the hydrolysis of chylomicron remnant-retinyl esters, in J774.1 cells. The cells were incubated with medium containing chylomicron remnant [(3)H]retinyl ester. Subcellular fractionation was used to separate early endosomes from late endosomes and lysosomes. About 26% and 80% of the total [(3)H]retinyl es...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1989
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1989.27.2.65